The formation or accumulation of bubbles in the detector cell can cause spikes and sawtooth noise or cause the baseline to fluctuate out of range. For example, in the case of high-pressure gradient mixing, flow lines are kept under high pressure after mixing, which suppresses bubble formation, but bubbles can form in the cell, where the pressure is near atmospheric. Section 2-3 described how the saturated solubility can decrease when solvents are mixed. Furthermore, bubbles can form in a gradient system, even without heating. If the column or detector cell is heated, bubbles can form more easily because the temperature of the solution passing through the cell is elevated and its pressure is close to atmospheric pressure.
0 kommentar(er)
